Online course basic analytical chemistry the university of tokyo via edx 0 151. The film must have a high boiling point mobile phase. This fourth edition of the classic guide for every user of gas chromatographic instrumentation is now updated to include such new topics as fast gc using narrow, short columns, electronic pressure control, and basic aspects of quantitative gas chromatography. This type of chromatography was primarily designed to evaluate volatile. In gc, the detector responds to the thermal conductivity of the gas stream or the ionisation of the gas as it passes through a flame. Hplc instrumentation, solvent reservoir in hplc, degasser in hplc, mixing valve in hplc, hplc pump, precolumn in hplc, guard column in hplc, sample injection in hplc, analytical column in hplc. Nptel mechanical engineering video lecture online study. Top 12 types of chromatographic techniques biochemistry. Thus the lectures will offer a substantial amount of information to the students. Flue gas analysis can be used to detect and to confirm and locate suspected condition of this nature. Develop a greater understanding of the chromatographic technique your laboratory uses. Bmc training provides a training course in applied gas chromatography in oil and gas training. The instrument used to perform gas chromatography is called a gas chromatograph or aerograph, gas separator. Chromatography definitionchromatography is defined as physicalmethod of separation, in which themixture of analytes is separated using twophases, one is stationary phase and otheris mobile phase which percolates throughthe stationary phase.
Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase stable phase is separating from each other while moving with the aid of a mobile phase. By the end of this course, you will feel comfortable understanding any modern gc. The stationary phase is a liquid layer supported over a stationary phase while the mobile phase is an inert and stable gas. Gas chromatography is a method of chemical analysis performed by separating and examining vaporized compounds. The power of chromatography comes from its ability to separate a mixture of compounds, or analytes, and. Nptel syllabus natural dyes video course course outline dyeing fabric is an ancient art.
Chapter 1 introduction, chromatography theory, and instrument calibration 1. The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column a homage to the fractionating column used in distillation. In column chromatography, mobile phase flows through the packed column, while in thin layer chromatography, mobile phase moves by capillary action. Gas chromatography differs from other forms of chromatography in that the mobile phase is a gas and the components are separated as vapors it is thus used to separate and detect small molecular weight compounds in the gas phase.
Nptel syllabus analytical technologies in biotechnology video course course outline microscopy radioisotope techniqueschromatographic methodselectrophoresiscentrifugation techniquesspectroscopic techniques polymerase chain reactionpcr, dna sequencing, elisa. Nptel provides elearning through online web and video courses various streams. Gas chromatography continues to be one of the most widely used analytical techniques, since its applications today expand into fields such as biomarker research or metabolomics. Sfc combines the advantages of gc and hplc thanks to the superior properties of supercritical fluids. Analytical chemistry inorganic chemistry medicinal chemistry organic chemistry. Chromatography not only separates out your target molecule, it concentrates it and offers a variety of downstream applications as a result. Practical gas chromatography a comprehensive reference. These courses often include a laboratory component and teach you how to use gas chromatography machines. Supercritical fluid chromatography is one of the most important column chromatography methods after gas chromatography gc and highperformance liquid chromatography hplc.
This postgraduate certificate in advanced chromatographic techniques was developed based on an analysis of the importance of the pharmaceutical and related industries in ireland and within the southeast region and also using information generated from th. However, with hplc, its a more advanced technique in that youre working with very, very small quantities, and the detector in the machine is much more sensitive. Explore the full program of hplc, gc and sample prep training courses for 2020. Classes specifically in gas chromatography are rare, but this subject is commonly taught in broader chemistry courses.
Chromatography is a separation process that is achieved by distributing the components of a mixture between two phases, a stationary phase and a mobile phase. A wide range of chromatographic procedures makes use of differences in size, binding affinities, charge, and other properties. Introduction to gas chromatography course and its objectives the overwhelming response to the hplc free elearning course has encouraged me to move ahead with the other courses announced earlier. Nevertheless, the material should be useful as an overview of the subject for those. Here are the links to the free online e course on gc. The use of flue gas analysis to promote efficiency are varied and many. Advanced membrane technology and applications by n n li wiley. The course provides the fundamentals to understand the techniques and instrumentation involved in this powerful analytical tool. Semester wise suggested reading for civil engineering. Chromatography biochemistry of medics 1 slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Gas chromatography laboratory training courses on hplc.
In gas chromatography, the moving phase or mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. Gas chromatography courses teach you how to use gas to separate different elements in a chemical mixture. Nptel syllabus novel separation processes video course. Learn all you need to know about polymer chemistry through a novel. Learn more about the different separation techniques and the chromatography solutions that thermo fisher scientific offers. Chromatography is a field of chemistry with many practical applications, such as in forensics, environmental testing, explosives detection and other laboratory technology fields. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Nptel material is available for free on nptel website. Gas chromatography or gas liquid chromatography is a technique applied for separation, identification and quantification of components of a mixture of organic compounds by selective partitioning between the stationary phase and mobile phase inside a column followed by sequential elution of separated components. Ashwani k sharma,department of biotechnology,iit roorkee. If you are not able to download lectures due to bandwidth constraints, you can obtain the courses in dvds by paying a nominal fee to cover the cost of storage media and distribution. Gas chromatography principle, instrumentation and method. This new practical textbook enables the reader to make full use of gas chromatography. Lecture 35 introduction to chromatography i by nptel iit.
Learn for free, pay a small fee for exam and get a certificate. Chromatography definition, principle, types, applications. This hazard can be present wherever combustible gases or vapors are in contact with a substance which will support combustion and the properties are in the explosive range. For the love of physics walter lewin may 16, 2011 duration.
Gain practical laboratory experience in gas chromatography. This article throws light upon the twelve types of chromatographic techniques used in biochemistry. If you continue browsing the site, you agree to the use of cookies on this website. Analytical technologies in biotechnology online course. Chapter 1 introduction, chromatography theory, and. Lecture notes polymer physics materials science and. Join our civil and construction engineering training courses, electrical and power engineering training courses and. Gas chromatography chemical engineering, iit bombay. Postgraduate certificate in advanced chromatographic. The modern analytical techniques with high accuracy like high performance liquid chromatography hplc and gas chromatography gc is extensively discussed in the book to get the understanding of. The factors effective on this separation process include molecular characteristics related to adsorption liquid. Gas chromatography runs on the principle of pa rtition chromatography for separation of components. Like the video and subscribe to channel for more updates. The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column a homage to the fractionating column used in.
Mudakavi,department of chemical engineering, iisc bangalore. Search for newer sources is of continued interest to dyers. The last kind of chromatography is gas chromatography. Gas chromatography classification according to the force of separation 1 adsorption chromatography. This is essentially the same as the basic column chromatography that you see there in yellow. These include column chromatography, high performance liquid. Find materials for this course in the pages linked along the left.
Gc online training laboratory training courses on hplc. Nptel syllabus analytical technologies in biotechnology. Chemical analysis of hazardous materials, environmental samples, inorganic, organic and biomaterials at trace and ultra trace quantities. Nptel, online courses and certification, learn for free. The troubleshooting and maintenance guide for gas chromatographers.